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Separate tumor cells from healthy cells by gradient magnetic separation method

The magnetic gradient separation‐based strategy described in the present study is a simple, fast, and feasible method for targeted separation of tumor cell subpopulations, and shows great

  • Vertical Magnetic Separation of Circulating Tumor Cells
    Vertical Magnetic Separation of Circulating Tumor Cells

    Efficient isolation and genetic analysis of circulating tumor cells (CTCs) from cancer patients' blood is a critical step for clinical applications using CTCs. Here, we report a novel CTC-isolation method and subsequent genetic analysis. CTCs from the blood were complexed with magnetic beads coated

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  • Technologies for circulating tumor cell separation from
    Technologies for circulating tumor cell separation from

    May 14, 2019 After tumor cells are captured on the SB microfilters, it is possible to separate the top and bottom parylene-C membranes to access the captured cells. Using multiple cancer cell lines spiked in healthy donor blood for analysis, the SB microfilter showed a capture efficiency of 78–83%, cell viability of 71–74%, and tumor cell enrichment of

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  • High depletion of breast cancer cells from the peripheral
    High depletion of breast cancer cells from the peripheral

    This method is based on cell type-specific interaction of living cells with Carboxymethyl Dextrane (CMD) coated magnetic nanoparticles. We have developed a mild flow separation method using CMD-coated magnetic nanoparticles (core size ca. 25 nm) along with a low-field gradient magnetic separator and an external separation column (blood bag)

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  • Isolation and Analysis of Tumor Cell Subpopulations Using
    Isolation and Analysis of Tumor Cell Subpopulations Using

    Sep 09, 2020 Sep 09, 2020 The magnetic gradient separation‐based strategy described in the present study is a simple, fast, and feasible method for targeted separation of tumor cell subpopulations, and shows great potential for clinical study

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  • Novel Automated Blood Separations Validate Whole Cell
    Novel Automated Blood Separations Validate Whole Cell

    Jul 22, 2011 Jul 22, 2011 Lymphocytes for biomarker assays from Ficoll versus a magnetic method. In order to detect rare lymphocyte populations, it is critical that the separation method yields healthy cells of sufficiently high purity. (A) Schematic depiction of magnetic and gradient methods

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  • Screening System using High Gradient Magnetic
    Screening System using High Gradient Magnetic

    In contrast, magnetic separation of cellular organelles is much less common than magnetic cell/bacteria/protein separation and often used only in the field of basic biology. As described in the following sections, endosomes [2–6], exosomes [7–12] and mitochondria [13–16] have been successfully isolated using the magnetic separation

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  • Healthy cells in = good data out - Fisher Sci
    Healthy cells in = good data out - Fisher Sci

    Choosing the right cell separation method is therefore critical to your downstream experiments. Dynabeads are designed with an inert coating to eliminate the risk that any unwanted material such as iron is left in the sample after separation. In addition, since the cells

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  • Magnetic cell separation | Cell separation | Miltenyi
    Magnetic cell separation | Cell separation | Miltenyi

    Following cell separation, both MicroBeads and REAlease Biotin Complex can be gently removed, leaving the cells bead- and label-free for further cell separation steps. REAlease Technology allows for magnetic cell isolation by positive selection of target cells and subsequent removal of any beads and labels from the cells

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  • Differential interaction of magnetic nanoparticles with
    Differential interaction of magnetic nanoparticles with

    Jan 24, 2006 Jan 24, 2006 Purpose: The separation of tumor cells from healthy cells is a vital problem in oncology and hematology, especially from peripheral blood.Magnetic assisted cell sorting (MACS) is a possibility to fulfill these needs. Methods: Tumor cell lines and leukocytes from peripheral blood were incubated with carboxymethyl dextran-coated magnetic nanoparticles under various conditions and separated

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  • Microfluidic, marker-free isolation of circulating tumor
    Microfluidic, marker-free isolation of circulating tumor

    A greater residence time within the magnetic gradient (lower flow rate) will also increase magnetic sensitivity, but at the expense of throughput. The CTC-iChip performs magnetic separation in two serial stages (see Fig. 4d for overall schematic and Fig. 5 for magnetophoretic design). In the first stage, cells travel at ~50 mm/s, and they are

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  • US4910148A - Magnetic separation of magnetized particles
    US4910148A - Magnetic separation of magnetized particles

    A method and device are provided for separating magnetized particles from biological fluids. Cells such as cancer cells coated with magnetized particles can be separated from uncoated healthy cells. A fluid mixture of cancer cells, healthy cells and magnetizable particles is introduced into a container such as a disposable blood bag which is attached in a cassette on an underlying plane

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  • AU2011208382B2 - Magnetic separation of rare cells
    AU2011208382B2 - Magnetic separation of rare cells

    A magnetic separation system configured to separate with high qualitative and quantitative yield magnetized cells from cell mixtures, comprising at least one electromagnet structured to generate a magnetic field flux about a plurality of separation zones and sufficient to attract a majority of the magnetized cells in the mixture, and a pump to drive the cell mixture at a controlled flow rate

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  • Immunomagnetic separation in a novel cavity-added
    Immunomagnetic separation in a novel cavity-added

    Oct 01, 2021 The manipulation and separation of circulating tumor cells (CTCs) in continuous fluidic flows play an essential role in various biomedical applications, particularly the early diagnosis and treatment of diseases. Recent advances in magnetic bead development have provided promising solutions to the challenges encountered in CTC manipulation and isolation. In this study, we proposed

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